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KMID : 0613820100200071005
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Journal of Life Science
2010 Volume.20 No. 7 p.1005 ~ p.1011
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Interaction of GAT1 with Ubiquitin-Specific Protease Usp14 in Synaptic Terminal
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Seog Dae-Hyun
Jang Won-Hee
Yea Sung-Su
Moon Il-Soo
Park Yeong-Hong
Kim Sang-Jin
Joung Young-Ju
Kim Moo-Seong
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Abstract
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γ-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the central nervous system. GABA transporters (GATs) control extracellular GABA levels by reuptake of released GABA from the synaptic cleft. However, how GATs are regulated has not yet been elucidated. Here, we used the yeast two-hybrid system to identify the specific binding protein(s) that interacts with the carboxyl (C)-terminal region of GAT1, the major isoform in the brain and find a specific interaction with the ubiquitin-specific protease 14 (Usp14), a deubiquitinating enzyme. Usp14 protein bound to the tail region of GAT1 and GAT2 but not to other GAT members in the yeast two-hybrid assay. The C-terminal region of Usp14 is essential for interaction with GAT1. In addition, these proteins showed specific interactions in the glutathione S-transferase (GST) pull-down assay. An antibody to GAT1 specifically co-immunoprecipitated Usp14 from mouse brain extracts. These results suggest that Usp14 may regulate the number of GAT1 at the cell surface.
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KEYWORD
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γ-aminobutyric acid, γ-aminobutyric acid transporter, ubiquitin, protein-protein interaction, deubiquitinating enzyme
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